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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidithiobacillus ferrooxidans N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP478866AWH
CSB-EP478866AWH
CSB-BP478866AWH
CSB-MP478866AWH
CSB-EP478866AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP478867AWH
CSB-EP478867AWH
CSB-BP478867AWH
CSB-MP478867AWH
CSB-EP478867AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP478868AWH
CSB-EP478868AWH
CSB-BP478868AWH
CSB-MP478868AWH
CSB-EP478868AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP478869AWH
CSB-EP478869AWH
CSB-BP478869AWH
CSB-MP478869AWH
CSB-EP478869AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Chaperone protein hscA homolog (hscA), partial CSB-YP478870AWH
CSB-EP478870AWH
CSB-BP478870AWH
CSB-MP478870AWH
CSB-EP478870AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S21 (rpsU) CSB-YP478871AWH
CSB-EP478871AWH
CSB-BP478871AWH
CSB-MP478871AWH
CSB-EP478871AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP478872AWH
CSB-EP478872AWH
CSB-BP478872AWH
CSB-MP478872AWH
CSB-EP478872AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Sugar fermentation stimulation protein homolog (sfsA) CSB-YP478873AWH
CSB-EP478873AWH
CSB-BP478873AWH
CSB-MP478873AWH
CSB-EP478873AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP478874AWH
CSB-EP478874AWH
CSB-BP478874AWH
CSB-MP478874AWH
CSB-EP478874AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP478875AWH
CSB-EP478875AWH
CSB-BP478875AWH
CSB-MP478875AWH
CSB-EP478875AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Protein ApaG (apaG) CSB-YP478876AWH
CSB-EP478876AWH
CSB-BP478876AWH
CSB-MP478876AWH
CSB-EP478876AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S6 (rpsF) CSB-YP478877AWH
CSB-EP478877AWH
CSB-BP478877AWH
CSB-MP478877AWH
CSB-EP478877AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP478878AWH
CSB-EP478878AWH
CSB-BP478878AWH
CSB-MP478878AWH
CSB-EP478878AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP478879AWH
CSB-EP478879AWH
CSB-BP478879AWH
CSB-MP478879AWH
CSB-EP478879AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP478880AWH
CSB-EP478880AWH
CSB-BP478880AWH
CSB-MP478880AWH
CSB-EP478880AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP478881AWH
CSB-EP478881AWH
CSB-BP478881AWH
CSB-MP478881AWH
CSB-EP478881AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP478882AWH
CSB-EP478882AWH
CSB-BP478882AWH
CSB-MP478882AWH
CSB-EP478882AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Bifunctional protein GlmU (glmU) CSB-YP478883AWH
CSB-EP478883AWH
CSB-BP478883AWH
CSB-MP478883AWH
CSB-EP478883AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP478884AWH
CSB-EP478884AWH
CSB-BP478884AWH
CSB-MP478884AWH
CSB-EP478884AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Phosphoglycerate kinase (pgk) CSB-YP478885AWH
CSB-EP478885AWH
CSB-BP478885AWH
CSB-MP478885AWH
CSB-EP478885AWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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